热球菌HJ21高温α-淀粉酶基因克隆、表达及性质研究          下载免费PDF全文

王淑军  吕明生  秦松  陆兆新  房耀维  邓祥元  林谦  刘红飞 《海洋学报》2011,33(3):158-164

通过简并引物扩增热球菌(Thermococcus siculi)HJ21高温α-淀粉酶保守区域之间的序列,再利用Site-finding技术获得两端未知序列.构建了在N端添加了His6标签的表达载体pEt-28a-His6-THJA后转化E.coli,在IPTG诱导下表达.进一步纯化后SDS-PAGE电泳检测达到电泳纯...  相似文献   

A spectral mixture model analysis of the Kuroshio variability and the water exchange between the Kuroshio and the East China Sea   总被引：1，自引：0，他引：1  

宋军  薛惠洁  鲍献文  吴德星  柴扉  施磊  姚志刚  王勇智  南峰  万凯 《中国海洋湖沼学报》2011,29(2):446-459

For understanding more about the water exchange between the Kuroshio and the East China Sea,We studied the variability of the Kuroshio in the East China Sea(ECS) in the period of 1991 to 2008 using a three-dimensional circulation model,and calculated Kuroshio onshore volume transport in the ECS at the minimum of 0.48 Sv(1 Sv ;106 m3/s) in summer and the maximum of 1.69 Sv in winter.Based on the data of WOA05 and NCEP,The modeled result indicates that the Kuroshio transport east of Taiwan Island decreased since 2000.Lateral movements tended to be stronger at two ends of the Kuroshio in the ECS than that of the middle segment.In addition,we applied a spectral mixture model(SMM) to determine the exchange zone between the Kuroshio and the shelf water of the ECS.The result reveals a significantly negative correlation(coefficient of-0.78) between the area of exchange zone and the Kuroshio onshore transport at 200 m isobath in the ECS.This conclusion brings a new view for the water exchange between the Kuroshio and the East China Sea.Additional to annual and semi-annual signals,intra-seasonal signal of probably the Pacific origin may trigger the events of Kuroshio intrusion and exchange in the ECS.  相似文献   

Application of stable isotope techniques in studies of carbon and nitrogen biogeochemical cycles of ecosystem   总被引：1，自引：1，他引：0  

Zhigao Sun  Xiaojie Mou  Xinhua Li  Lingling Wang  Hongli Song  Huanhuan Jiang 《中国地理科学(英文版)》2011,21(2):129-148

Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.  相似文献   

Numerical study on the bifurcation of the North Equatorial Current     

Yulong Liu  Qi Wang  Jun Song  Xiande Zhu  Xiaoqing Gong  Fang Wu 《中国海洋大学学报(英文版)》2011,10(4):305-313

A 1.5-layer reduced-gravity model forced by wind stress is used to study the bifurcations of the North Equatorial Current(NEC).The authors found that after removing the Ekman drift,the modelled circulations can serve well as a proxy of the SODA circulations on the σθ=25.0 kg m～-3 potential density surface based on available long-term reanalysis wind stress data.The modelled results show that the location of the western boundary bifurcation of the NEC depends on both zonal averaged and local zero wind stress curl latitude.The effects of the anomalous wind stress curl added in different areas are also investigated and it is found that they can change the strength of the Mindanao Eddy(ME),and then influence the interior pathway.  相似文献   

空间规划决策支持系统在区域主体功能区划分中的应用   总被引：4，自引：1，他引：3  

宗跃光  张晓瑞  何金廖  薛松 《地理研究》2011,30(7):1285-1295

按照国家“十一五”规划中关于主体功能区划分的要求,将英国Pearce教授的自然资本不能减少的强可持续发展生态阈值理论与主体功能区划分的生态评价技术相结合,以潜力一阻力模型为基础,首次通过构建可开发度指数（PDI）和四力模型,创建一套主体功能区划分的综合指标体系,包括生态敏感性、环境压力、社会经济发展潜力和自然资源潜力等...  相似文献   

黔南州主要农作物病虫害监测预警专家系统的构建与实现     

宋启堃  郑松  宋彦棠 《云南地理环境研究》2011,23(1):31-33

通过对1981～2010年30年的贵州黔南地区气象资料与植保病虫害资料的对比研究,得出了农作物病虫害发生的一系列气象条件指标,研究结果表明,作物病虫害的发生与气象条件有密切的关系,主要与气温、相对湿度、日照和降水量有关,而不同种类的病虫害发生的气象条件指标不尽相同。在指标结论的基础上,利用VB.NET设计并开发了黔南州主要农作物病虫害监测预警专家系统,将气象资料实时入库监测分析,达到某种病虫害发生条件即预警,从而实现了农作物病虫害监测预警专家系统的构建。  相似文献   

大尺度水循环模拟系统不确定性研究进展(英文)   总被引：2，自引：1，他引：1  

宋晓猛  占车生  孔凡哲  夏军 《地理学报(英文版)》2011,21(5):801-819

The regional hydrological system is extremely complex because it is affected not only by physical factors but also by human dimensions.And the hydrological models play a very important role in simulating the complex system.However,there have not been effective methods for the model reliability and uncertainty analysis due to its complexity and difficulty.The uncertainties in hydrological modeling come from four important aspects:uncertainties in input data and parameters,uncertainties in model structure,uncertainties in analysis method and the initial and boundary conditions.This paper systematically reviewed the recent advances in the study of the uncertainty analysis approaches in the large-scale complex hydrological model on the basis of uncertainty sources.Also,the shortcomings and insufficiencies in the uncertainty analysis for complex hydrological models are pointed out.And then a new uncertainty quantification platform PSUADE and its uncertainty quantification methods were introduced,which will be a powerful tool and platform for uncertainty analysis of large-scale complex hydrological models.Finally,some future perspectives on uncertainty quantification are put forward.  相似文献   

Re–Os and U–Pb Geochronology of Porphyry and Skarn Types Copper Deposits in Jilin Province,NE China          下载免费PDF全文

Yong Zhang  Shu–Wen Xing  Quan–Heng Song  Yan Wang  Zheng–Tao Yu  Xiao–Hui Du  Yu–Bo Ma  Zeng–Jie Zhang 《Resource Geology》2015,65(4):394-404

Jilin Province in NE China lies on the eastern edge of the Xing–Meng Orogenic Belt. Mineral exploration in this area has resulted in the discovery of numerous large, medium, and small sized Cu, Mo, Au, and Co deposits. To better understand the formation and distribution of both the porphyry and skarn types Cu deposits of the region, we examined the geological characteristics of the deposits and applied zircon U–Pb and molybdenite Re–Os isotope dating to constrain the age of the mineralization. The Binghugou Cu deposit yields a zircon U–Pb age for quartz diorite of 128.1 ± 1.6 Ma; the Chang'anpu Cu deposit yields a zircon U–Pb age for granite porphyry of 117.0 ± 1.4 Ma; the Ermi Cu deposit yields a zircon U–Pb age for granite porphyry of 96.8 ± 1.1 Ma; the Tongshan Cu deposit yields molybdenite Re–Os model ages of 128.7 to 130.2 Ma, an isochron age of 129.0 ± 1.6 Ma, and a weighted mean model age of 129.2 ± 0.7 Ma; and the Tianhexing Cu deposit yields molybdenite Re–Os model ages of 113.9 to 115.2 Ma, an isochron age of 114.7 ± 1.2 Ma, and a weighted mean model age of 114.7 ± 0.7 Ma. The new ages, combined with existing geochronology data, show that intense porphyry and skarn types Cu mineralization was coeval with Cretaceous magmatism. The geotectonic processes responsible for the genesis of the Cu mineralization were probably related to lithospheric thinning. By analyzing the accumulated molybdenite Re–Os, zircon U–Pb, and Ar–Ar ages for NE China, it is concluded that the Cu deposits formed during multiple events coinciding with periods of magmatic activity. We have identified five phases of mineralization: early Paleozoic (~476 Ma), late Paleozoic (286.5–273.6 Ma), early Mesozoic (~228.7 Ma), Jurassic (194.8–137.1 Ma), and Cretaceous (131.2–96.8 Ma). Although Cu deposits formed during each phase, most of the Cu mineralization occurred during the Cretaceous.  相似文献   

羌塘盆地羌资5井二叠系展金组烃源岩基本特征   总被引：2，自引：1，他引：1  

曹竣锋  宋春彦  付修根  陈明  郑博  孙伟 《海相油气地质》2015,(2)

前人对羌塘盆地的烃源岩研究资料主要来源于中生界地表露头,少见古生界的。对羌资5井二叠系展金组烃源岩的钻井岩心研究表明,其有机碳含量高,平均值达到1.15%,属于好烃源岩。干酪根显微组分主要为Ⅲ型,其次为Ⅱ2型;有机质主要来自于海相低等水生生物,热演化已达到成熟阶段。烃源岩具较好的生气能力。认为井下烃源岩样品虽显示其处于有机质演化成熟阶段,但因受后期构造抬升的影响,推测二叠系深部未知的烃源岩可能尚处于高成熟—过成熟阶段。羌塘盆地二叠系具有较好的勘探前景,油气勘探可向深部古生界开展。  相似文献   

巢湖地区二叠系栖霞组和三叠系南陵湖组石灰岩生物标志物特征与生烃潜力——以平顶山和马家山剖面为例     

宋换新  志刚  包建平 《海相油气地质》2015,(2)

对安徽巢湖地区平顶山和马家山剖面下二叠统栖霞组和下三叠统南陵湖组进行了地层沉积特征及暗色石灰岩生烃潜力的综合分析,并对这两个层位的样品进行了饱和烃生物标志物特征研究。结果表明,两个层位的石灰岩都属于有效烃源岩,均具有一定的生烃潜力。南陵湖组石灰岩饱和烃以高丰度的长链三环萜烷、重排藿烷、重排甾烷为特征,表明其形成于偏弱氧化的沉积环境。栖霞组沉积期水体能量相对较低,受陆源物质影响明显,栖霞组石灰岩萜烷系列为常规的分布模式,但其甾烷成熟度参数明显低于平衡值,认为高的热演化程度是造成栖霞组石灰岩20S/(20S+20R)-ααα-C29甾烷值和ββ/(ββ+αα)-C29甾烷值"倒转"的主要原因。  相似文献